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Products >  Molecular_Biology >  Nucleic_Acids >  Vectors >  pUC18-19_Type_Vectors

pUC18 Plasmid and pUC19 Plasmid

The pUC18 plasmid and pUC19 plasmid enable successful cloning of larger DNA fragments than the M13 mp18 RF Phage Vector. Because these cloning vectors contain a multiple cloning site at the lacZ' region, recombinant plamids can be verified via blue/white colony screening using agar plates containing IPTG and X-Gal. Expression of target DNA is enabled by the presence of a lac promoter in the cloning vectors.

The pUC18 plasmid and pUC19 plasmid are suitable for dideoxy DNA sequencing using M13 primers. The Deletion Kit for Kilo-Sequencing may be used to easily sequence pUC18 Plasmid and pUC19 Plasmid containing large DNA insertions.

At-A-Glance Documents Images & Data

Applications

  • Cloning and expression of target genes 
  • DNA sequencing using M13 primers
  • Large DNA insert sequencing using Takara's Deletion Kit for Kilo-Sequencing 

Purity

  • >70% double-stranded, covalently closed circular form I (RF I) DNA.
  • Multiple cloning site sequence verified by dideoxy DNA sequencing.  
  • Restriction site verified by restriction enzyme cleavage. 

Storage

–20°C

Concentration

250–1,000 µg/mL

Form

10 mM Tris-HCl (pH 8.0), 1 mM EDTA

Size

2,686 bp

GenBank

Entry NameAccession No.
pUC18SYNPUC18CVL09136
pUC19SYNPUC19CVL09137




 
 
Products
Cat. # Product Package Size Price # of Units Select
3218 pUC 18 DNA 25 ug $47.00
3219 pUC 19 DNA 25 ug $47.00
 

 

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Cat. # Product Package Size Price License # of Units Select
3340 Chaperone Plasmid Set 1 Set $225.00 License Statements
6030 Deletion Kit for Kilo-Sequencing 5 Rxns $169.00  
 

 



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