This DNA is a plasmid vector suitable for DNA sequencing by dideoxy method. This DNA may allow successful cloning of DNA fragment longer than the case of M13 phage vector used. Since it has multi-cloning sites on lacZ' region, any DNA insertion into this vector can be easily verified using plates containing IPTG and X-Gal. Moreover, expression of the cloned DNA can be performed using a lac promoter. M13 primer is available for DNA sequencing.
Using Deletion Kit for Kilo-Sequencing, DNA sequencing of the cloned DNA with kilo-bases can also be performed.
