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Products >  Molecular_Biology >  Nucleic_Acids >  Vectors >  T-vectors_pMD19(simple)-20

T-Vectors pMD20 and pMD19 (Simple) for TA Cloning

pMD20 and pMD19 (Simple) T-Vectors are linearized PCR cloning vectors (EcoRV cleaved) that have single 3’-terminal thymidine residues (dT) at both ends. The T-overhangs at the cloning site improve the efficiency of ligation of PCR products that contain dA-overhangs, such as those amplified with TaKaRa Taq, TaKaRa Ex Taq, TaKaRa LA Taq, or SpeedSTAR HS. The inclusion of lacZ in these vectors allows blue/white screening for the detection of successful ligations.

pMD19 (Simple) is derived from pUC19 and has deletions of all of the restriction enzyme sites in the multiple-cloning site (MCS). Therefore, after cloning, restriction enzyme digestion analysis of the PCR insert is possible.

pMD20 retains the MCS of pUC19. In addition, this vector contains the SP6 promoter upstream of MCS. Therefore, the inserted DNA fragments can be transcribed by SP6 RNA Polymerase.

Both vectors are supplied with a Control Insert (500 bp) DNA for use as a positive control.

Note: The ends of PCR products amplified with PrimeSTAR enzymes are blunt. In this case, adding dA to the 3' ends before ligation into pMD19 or pMD20 is recommended.

At-A-Glance Documents Images & Data

Features

  • Concentration: 50 ng/µL
  • Amount: 1 µg
  • Vector Size: pMD20 2,736 bp; pMD19 (Simple) 2,692 bp

Applications

  • TA Cloning
  • PCR Cloning
  • SP6 expression (pMD20 only)

Components

  • pMD20 or pMD19 (Simple) (50 ng/μL)
  • Control Insert (50 ng/μL)*

* 500 bp DNA fragment with a 3' dA overhang

Storage

-20°C




 
 
Products
Cat. # Product Package Size Price # of Units Select
3270 T-Vector pMD20 1 ug $80.00
3271 T-Vector pMD19 (Simple) 1 ug $80.00
 

 

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