pMD19 (Simple) is derived from pUC19 and has deletions of all of the restriction enzyme sites in the multiple-cloning site (MCS). Therefore, after cloning, restriction enzyme digestion analysis of the PCR insert is possible.
pMD20 retains the MCS of pUC19. In addition, this vector contains the SP6 promoter upstream of MCS. Therefore, the inserted DNA fragments can be transcribed by SP6 RNA Polymerase.
Both vectors are supplied with a Control Insert (500 bp) DNA for use as a positive control.
Note: The ends of PCR products amplified with PrimeSTAR enzymes are blunt. In this case, adding dA to the 3' ends before ligation into pMD19 or pMD20 is recommended.