This DNA is a plasmid vector suitable for DNA sequencing by dideoxy method. This DNA may allow successful cloning of DNA fragment longer than the case of M13 phage vector used. Since it has multi-cloning sites on lacZ' region, any DNA insertion into this vector can be easily verified using plates containing IPTG and X-Gal. Moreover, expression of the cloned DNA can be performed by using of lac promoter. M13 primer is available for DNA sequencing.
Using Deletion Kit for Kilo-Sequencing, DNA sequencing of the cloned DNA with kilo-bases can also be performed.
Those are pUC type of cloning vector plasmid, pHSG298 and pHSG299 possessing kanamycin resistancy as selective markers, while pHSG396, pHSG397, pHSG398 and pHSG399 possessing chloramphenicol resistancy, respectively. pHSG298 and pHSG398 contain the consensus multi-cloning sites as same as pUC18, while pHSG299 and pHSG399 contain the consensus one as the same as pUC19 (even though Hind III and Sma I sites in pHSG298 and pHSG299 are not available). Multi-cloning sites of pHSG396 and pHSG397 are partially converted sites of pHSG399.
