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Products >  Molecular_Biology >  Nucleic_Acids >  Vectors >  Chloramphenicol_Resistant_pUC_Vectors

Chloramphenicol Resistant pUC Vectors

The chloramphenicol resistant pUC vectors pHSG396 and pHSG398 contain the chloramphenicol resistance gene, the pMB1-derived origin of replication (ori), and the beta-galactosidase coding gene lacZ. These chloramphenicol resistant pUC vectors also contain a pUC18-derived multiple cloning site (MCS) within the lacZ gene, enabling recombinant clones to be verified through culture plates containing IPTG and X-Gal. High target gene expression is enabled by the presence of the lac promoter on both the pHSG396 and pHSG398 vectors.

Both pHSG 396 and pHSG 398 can be analyzed via dideoxy DNA sequencing using an M13 primer.  Large recombinant chloramphenicol resistant pUC vectors may be analyzed using Takara's Deletion Kit for Kilo-Sequencing (Cat. #6030).

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Features

  • Form: 10 mM Tris-HCl (pH 8.0), 1 mM EDTA
  • Concentration: 250–1,000 µg/mL

Applications

  • lac promoter-enabled target gene cloning and expression
  • DNA sequencing using an M13 primer
  • Long DNA sequencing using Takara's Deletion Kit for Kilo-Sequencing

Source

pUC18 vector

Purity

  • >70% double-stranded, covalently closed circular form (RF 1) DNA
  • Multiple cloning site sequence-verified via dideoxy DNA sequencing

Storage

–20°C

GenBank

               
VectorEntry NameAccession No.
pUC18SYNPUC18CVL09136
pHSG399SYNHSG399M19087*

*Except for the MCS, pHSG396 and pHSG398 have the same sequence as pHSG399.


Chain Length

  • pHSG396: 2,238 bp
  • pHSG398: 2,227 bp

References

1. Takeshita, S. et al. (1987) Gene 61:63-74.



 
 
Products
Cat. # Product Contents Size Price Units Select
3396 pHSG 396 DNA 25 ug $57.00
3398 pHSG 398 DNA 25 ug $57.00
 

 

Recommended Products
Cat. # Product Contents Size Price Units Select
6030 Deletion Kit for Kilo-Sequencing 5 Rxns $169.00
 

 

 
 

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