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Products >  Molecular_Biology >  Nucleic_Acids >  Linkers >  Phosphorylated_Linkers

Phosphorylated Linkers

Takara's phosphorylated linkers are cloning reagents that contain a phosphate group at their 5'-end, enabling them to be used directly in linker ligation during cloning experiments. The in-frame cloning of a target gene into a vector can be easily performed by using a phosphorylated linker length (e.g., 10 mer, 8 mer, 6 mer) that results in the desired reading frame.

At-A-Glance Documents Images & Data

Features

Sequence Information

 
Linker, pNot I (10 mer)pd(AGCGGCCGCT)
Linker, pPst I (8 mer)pd(GCTGCAGC)
Linker, pSma I (6 mer)pd(CCCGGG)

Purity

  • Confirmed by HPLC

Storage

–20°C (products ship at room temperature)

Notes

These products are provided as single-stranded oligonucleotides. For use in subsequent reactions, dissolve in a buffer such as TE, incubate at 70°C for 10 minutes and anneal by cooling slowly.

Activity 

  • Biological activity confirmed by self-ligation and restriction enzyme cleavage

    • Form

    Lyophilized

    References

    1. Smith, D.I. et al. (1976) Nucleic Acids Res. 3:343-353.

    2. Brown, N.L. & Smith, M. (1976) FEBS Letters 65:284-287.

    3. Roberts, R.J. (1985) Nucleic Acids Res. 13:r165-r200.


     
     
    Products
    Cat. # Product Package Size Price # of Units Select
    4967P Linker, pNot I, Phosphorylated (10 mer) 5,000 pmol $75.00
    4673P Linker, pPst I 5,000 pmol $57.00
    4585P Linker, pSma I, Phosphorylated 5,000 pmol $75.00
     

     


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