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Products >  Molecular_Biology >  Nucleic_Acids >  Linkers >  Phosphorylated_Linkers

Phosphorylated Linkers

Intended Use

  • Because the 5'-end of these linkers is phosphorylated, they can be directly used in ligations.
  • The cloning of a target gene into a vector can be easily performed matching the structural gene frame by selective use of linkers (8 mer, 10 mer, 12 mer).

At-A-Glance Documents

Features

Sequence Information

Linker, pApa I pd(GGGGCCCC)
Linker, pBamH (8 mer) pd(CGGATCCG)
Linker, pBamH (12 mer) pd(CCCGGATCCGGG)
Linker, pBgl II pd(CAGATCTG)
Linker, pCla I pd(CATCGATG)
Linker, pEcoR I (8 mer) pd(GGAATTCC)
Linker, pEcoR I (12 mer) pd(CCGGAATTCCGG)
Linker, pHind III (8 mer) pd(CAAGCTTG)
Linker, pKpn I pd(GGGTACCC)
Linker, pNco I (8 mer) pd(GCCATGGC)
Linker, pNde I (8 mer) pd(CCATATGG)
Linker, pNot I (8 mer) pd(GCGGCCGC)
Linker, pNot I (10 mer) pd(AGCGGCCGCT)
Linker, pPst I pd(GCTGCAGC)
Linker, pSal I pd(GGTCGACC)
Linker, pSma I pd(CCCGGG)
Linker, pSpe I pd(GACTAGTC)
Linker pSse 8387 I (pPst I) pd(CCTGCAGG)
Linker, pXba I pd(CTCTAGAG)
Linker, pXho I pd(CCTCGAGG)

Purity

  • Confirmed by HPLC
  • Biological activity is confirmed by self-ligation and restriction enzyme cleavage

Storage

–20°C (products ship at room temperature)

Notes

These products are single-stranded oligonucleotides. For use in subsequent reactions, dissolve in an appropriate buffer such as TE, incubate at 70°C for 10 minutes and then anneal by cooling slowly.

Form

Lyophilized


 
 
Products
Cat. # Product Package Size Price # of Units Select
4810P Linker, pBamH I, Phosphorylated (12 mer) 5,000 pmol $74.00
4610P Linker, pBamH I, Phosphorylated (8 mer) 5,000 pmol $56.00
4621P Linker, pBgl II, Phosphorylated 5,000 pmol $74.00
4630P Linker, pCla I, Phosphorylated 5,000 pmol $56.00
4840P Linker, pEcoR I, Phosphorylated (12 mer) 5,000 pmol $74.00
4640P Linker, pEcoR I, Phosphorylated (8 mer) 5,000 pmol $56.00
4660P Linker, pHind III, Phosphorylated (8 mer) 5,000 pmol $74.00
4967P Linker, pNot I, Phosphorylated (10 mer) 5,000 pmol $74.00
4966P Linker, pNot I, Phosphorylated (8 mer) 5,000 pmol $74.00
4673P Linker, pPst I 5,000 pmol $56.00
4680P Linker, pSal I, Phosphorylated 5,000 pmol $56.00
4585P Linker, pSma I, Phosphorylated 5,000 pmol $74.00
4983P Linker, pSse8387 I (pPst I), Phosphorylated 5,000 pmol $74.00
4693P Linker, pXba I, Phosphorylated 5,000 pmol $56.00
4694P Linker, pXho I 5,000 pmol $56.00
 

 


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