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Products >  Molecular_Biology >  Nucleic_Acids >  Linkers >  Nonphosphorylated_Linkers

Nonphosphorylated Linkers for Cloning

Nonphosphorylated linkers may be used as cloning tools for introducing restriction sites during ligation reactions. Takara's nonphosphorylated linkers for cloning are single-stranded oligonucleotides that introduce a restriction enzyme recognition site following ligation to the target DNA sequence.

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Features

Sequences

  • 4680A: d(GGTCGACC)
  • 4685A: d(GCCCGGGC)

Purity

  • Confirmed by HPLC
  • Biological activity confirmed by self ligation and restriction enzyme cleavage

Storage

–20°C. Product ships at room temperature.

Notes

These products are single-stranded oligonucleotides. For use in downstream applications, dissolve in an appropriate buffer such as TE, incubate at 70°C for 10 minutes and anneal by cooling slowly.

Form

Lyophilized




 
 
Products
Cat. # Product Contents Size Price Units Select
4680A Linker, Sal I 10,000 pmol $75.00
4685A Linker, Sma I 10,000 pmol $75.00
 

 

 
 

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