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Molecular Biology


Products >  Molecular_Biology >  Nucleic_Acids >  Linkers >  Nonphosphorylated_Linkers

Nonphosphorylated Linkers for Cloning

Nonphosphorylated linkers may be used as cloning tools for introducing restriction sites during ligation reactions. A nonphosphorylated linker consisting of a single-stranded oligonucleotide can be used to introduce a restriction enzyme recognition site following ligation to the target DNA sequence.

  At-A-Glance   Images & Data

Features

Sequence Information

Linker, Sma I (8 mer) d(GCCCGGGC)

Purity

  • Confirmed by HPLC
  • Biological activity confirmed by self ligation and restriction enzyme cleavage

Storage

–20°C. Product ships at room temperature.

Notes

These products are single-stranded oligonucleotides. For use in downstream applications, dissolve in an appropriate buffer such as TE, incubate at 70°C for 10 minutes and anneal by cooling slowly.

Form

Lyophilized




 
 
Products
Cat. # Product Size Price Units Select
4685A Linker, Sma I 10,000 pmol $75.00
 

 

 
 

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