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Products >  Molecular_Biology >  Nucleic_Acids >  Linkers >  Nonphosphorylated_Linkers

Nonphosphorylated Linkers

These products are single-stranded oligonucleotides used in cloning to introduce a restriction enzyme site following ligation to a target DNA fragment.
At-A-Glance Documents

Features

Sequences

  • 4710A: d(CCGGATCCGG)
  • 4673A: d(GCTGCAGC)
  • 4678A: d(CGAGCTCG)
  • 4680A: d(GGTCGACC)
  • 4685A: d(GCCCGGGC)

Purity

  • Confirmed by HPLC
  • Biological activity is confirmed by self ligation and restriction enzyme cleavage

Storage

–20°C. Product ships at room temperature.

Notes

These products are single-stranded oligonucleotides. For use in the subsequent reaction, dissolve in an appropriate buffer such as TE, incubate at 70°C for 10 minutes, and then anneal by cooling slowly.

Form

Lyophilized


 
 
Products
Cat. # Product Package Size Price # of Units Select
4710A Linker, BamH I (10 mer) 10,000 pmol $74.00
4680A Linker, Sal I 10,000 pmol $74.00
4685A Linker, Sma I 10,000 pmol $74.00
 

 


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