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Products >  Molecular_Biology >  Nucleic_Acids >  DNA >  pHY300PLK_DNA

E. coli-Bacillus Shuttle Vector pHY300PLK

The E.coli-Bacillus Shuttle Vector pHY300PLK is constructed from the E.coli-derived plasmid pACYC177 and the Streptococcus faecalis-derived plasmid pAMa1; as a result, the vector can be used to transform both E. coli and Bacillus subtillis. The E. coli-Bacillus Shuttle Vector DNA contains both the ampicillin resistance and tetracycline resistance genes as selective markers; while both of these resistance genes can be expressed in E. coli, only the tetracycline resistance gene can be expressed in B. subtillis. The pHY300PLK vector also contains a single cleavage site for restriction enzymes Bal I, BamH I, Ban I, Bgl I, Bgl II, Bst P I, EcoR I, EcoR V, Hind III, Hpa I, Sal I, Sma I, Pvu I and Xba I.

 

At-A-Glance Documents Images & Data

Vector size

4,870 bp

Source

Manufactured by Yakult Co., Ltd.

Purity

>70% double-stranded covalently closed circular form I (RF I) DNA.

Storage

–20°C. Supplied with the host strain Bacillus subtilis ISW 1214 (Glycerol stock, Storage : –80°C)

Form

10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA

References

1. Anagostopoulos, C. & Spizizen, J. (1961) J. Bacteriol. 81:741-746.

2. Ikawa, S. et al. (1981) Mol. Gen. Genet. 183:1-6.

3. Sadaie, Y. & Kada, T. (1983) J. Bacteriol. 153:813-821.

4. Ishiwa, H. & Tsuchida, N. (1984) Gene 32:129-134.

5. Ishiwa, H. & Shibahara, H. (1985) Jpn. J. Genet. 60:235-243.

Concentration

300–800 µg/mL

Transformation Efficiency

  • E. coli: >2 × 105/µg DNA
  • B. subtillis: >1 × 102/µg DNA (by electroporation)

Transformation efficiency of competent B. subtillis largely depends on the percentage of dimerized pHY300PLK vector. To increase transformation efficiency, first amplify pHY300PLK DNA with an E. coli recA+ strain.

Prior to electroporation of B. subtillis, amplify vector with an E.coli recA- strain to obtain monomeric pHY300PLK.

 




 
 
Products
Cat. # Product Package Size Price # of Units Select
3060 pHY300PLK DNA 10 ug $225.00
 

 


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