Chain Length 4,870 bp
Purity Contains over 70% double-strand covalently closed circular form I (RF I).
Storage –20°C. Supplied with Host strain: Bacillus subtilis ISW 1214 (Glycerol stock, Storage : –80°C)
Form 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA
Concentration 300–800 µg/mL
Transformation Efficiency - E. coli > 2 × 105/µg DNA
- B. subtillis > 1 × 102/µg DNA (electroporation method)
Transformation efficiency using competent cells by plasmid of B. subtillis largely depends on the contents of dimer. Therefore, to increase frequency of transformation, this DNA must be amplified in E. coli recA+ strain prior to transformation. In case of electroporation, use of the monomer plasmid is recommended.
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