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Products >  Molecular_Biology >  Nucleic_Acids >  DNA >  M13_mp18_RF_DNA

M13 mp18 RF Phage Vector

The M13 mp18 RF Phage Vector is suitable for M13 sequencing by the dideoxy sequencing method. Since the phage vector contains a multiple cloning site in the lacZ' region, recombinant vectors can be easily verified via blue/white colony screening using agar plates containing IPTG and X-Gal. The phage vector can also be directly used in M13 sequencing reactions because it is processed as a single-stranded DNA from phage particles. Takara's M13 primers (Cat. #3831, 3832 and 3830) are available for M13 sequencing. Takara's Deletion Kit for Kilo-Sequencing (Cat. # 6030) can be used  for sequencing of the cloned M13 mp18 RF phage vector DNA.
At-A-Glance Documents Images & Data

Purity

  • >70% double-stranded covalently closed circular form I (RF I) DNA.
  • Multiple cloning site integrity verified by dideoxy sequencing analysis.
  • EcoR I, Sac I, Kpn I, Sma I, BamH I, Xba I, Sal I, Pst I, Sph I and Hind III restriction sites verified by restriction enzyme analysis.

Storage

–20°C

Notes

Cloning of long DNA fragments (> 4 kb) using the M13 mp18 RF Phage Vector can result in some colony deletions.

Form

10 mM Tris-HCl (pH 8.0), 1 mM EDTA

References

1. Yanisch-Perron, C. et al. (1985) Gene 33:103-119.

2. Messing, J. (1983) Methods in Enzymology 101:20-78.

GenBank

  • Entry name: M13MP18
  • Accession No.: X02513

Concentration

250–1,000 µg/mL


 
 
Products
Cat. # Product Package Size Price # of Units Select
3118 M13 mp18 RF DNA 10 ug $75.00
 

 


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