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Products >  Molecular_Biology >  Nucleic_Acids >  DNA >  M13_mp18_RF_DNA

M13 mp18 RF Phage Vector

This DNA is a phage vector suitable for DNA sequencing by dideoxy method. Since it has multi-cloning sites on lacZ' region, any DNA insertion into this phage vector can be easily verified using soft-agar plates containing IPTG and X-Gal. Moreover, the cloned DNA can be directly used for DNA sequencing, because it is a single-strand DNA when isolated from the phage particle. M13 primer is available for DNA sequencing. Using Deletion Kit for Kilo-Sequencing, DNA sequencing of the cloned DNA with kilo-bases can also be performed.

At-A-Glance Documents Images & Data

Purity

  • Contains over 70% double-strand covalently closed circular form I (RF I).
  • It is confirmed that the multi-cloning site is well-maintained upon sequence analysis by dideoxy method.
  • It is confirmed that the vector contains a single cleavage site for EcoR I, Sac I, Kpn I, Sma I, BamH I, Xba I, Sal I, Pst I, Sph I and Hind III upon restriction analysis by the enzymes.

Storage

–20°C

Notes

When cloning for a long DNA fragment (e.g. above 4 to 5 kbp) using M13 phage vector, some deletion is often found during the culture.

Form

10 mM Tris-HCl (pH 8.0), 1 mM EDTA

GenBank

  • Entry name: M13MP18
  • Accession No.: X02513

Concentration

250–1,000 µg/mL




 
 
Products
Cat. # Product Package Size Price # of Units Select
3118 M13 mp18 RF DNA 10 ug $74.00
 

 



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