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Products >  Molecular_Biology >  Nucleic_Acids >  Adaptors

Adaptors

Molecular cloning using adaptors enables the quick addition of restriction enzyme recognized sequences without the need to generate specific primers and perform PCR. The EcoRI-NotI-BamHI Adaptor can be used for adaptor ligation projects that require the introduction of NotI and BamHI restriction sites. This adaptor has a 4-base 5' overhang corresponding to its EcoRI half-site in addition to internal NotI and BamHI sites. The EcoRI-NotI-BamHI Adaptor is provided as a double-stranded, annealed product and therefore can be used directly after being dissolved in sterilized water or TE buffer.

Sequence:

  • EcoRI-NotI-BamHI Adaptor: 5'-AATTCGGCGGCCGCGGATCC-3' annealed to 3'-GCCGCCGGCGCCTAGG-5' and containing a 5'-AATT overhang.
At-A-Glance Documents Images & Data Resources

Applications

  • Cohesive restriction site generation in blunt-ended DNA
  • Multiple restriction site generation in cloning vectors


Form

Lyophilized

References

1. Bahl, C.P. et al. (1978) Biochem. Biophys. Res. Commun. 81:695-703.

2. Rothstein, R.J. et al. (1979) Methods Enzymol. 68:98-109.

3. Tanabe, C. et al. (2003) Genes Chromosomes and Cancer 38(2):168–176.

4. Uemura, T. et al. (2002) Biochem. Biophys. Res. Comm. 296:492–496.


 
 
Products
Cat. # Product Package Size Price # of Units Select
4510 Adaptor, EcoR I-Not I-BamH I 1,000 pmol $57.00
 

 


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