Molecular cloning using adaptors enables the quick addition of restriction enzyme recognized sequences without the need to generate specific primers and perform PCR. The EcoRI-NotI-BamHI Adaptor can be used for adaptor ligation projects that require the introduction of NotI and BamHI restriction sites. This adaptor has a 4-base 5' overhang corresponding to its EcoRI half-site in addition to internal NotI and BamHI sites. The EcoRI-NotI-BamHI Adaptor is provided as a double-stranded, annealed product and therefore can be used directly after being dissolved in sterilized water or TE buffer.
EcoRI-NotI-BamHI Adaptor: 5'-AATTCGGCGGCCGCGGATCC-3' annealed to 3'-GCCGCCGGCGCCTAGG-5' and containing a 5'-AATT overhang.