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Products >  Molecular_Biology >  Molecular_Biology_Reagents >  General >  Westase

Westase Enzyme

Westase enzyme enables fungal and yeast cell wall digestion via lysis of beta-glucanase (beta-1,6 glucanase and beta-1,3-glucanase) bonds for experimental protocols including spheroplast generation, immunofluorescence, protein purification and transformation. Westase enzyme works well for beta-glucanase lysis in Ascosporogenous type yeasts such as Saccharomyces cerevisiae. It can also be used for yeast cell wall digestion of fission yeasts such as Schizosaccharomyces pombe and Basidiosporogenous since they cannot be protoplasted with simple Zymolyase treatment.

At-A-Glance Documents Images & Data

Applications

  • Yeast cell wall digestion
  • Beta-glucanase bond lysis 

Source

Westase enzyme is prepared from Streptomyces rochei DB-34 liquid cell culture supernatant.

Storage

4°C (dry condition)

Notes

Manufactured by Ozeki Corporation.

Form

Lyophilized powder (containing celite as the excipient).

Definition of Activity

Beta-1,6 glucanase activity: One unit is defined as the amount of enzyme required to release 1 micromole of reduced sugar from 10 mg/mL Pustulan solution in 1 min. at 37°C (pH 6.0).

Lytic activity: One unit is defined as the amount of enzyme required to decrease 660 nm absorbance by 1% in 1 min. at 30°C (pH 6.0) when using Cryptococcus albidus IFO 0612 cell wall fraction as a substrate.

Specific Activity

  • Beta-1,6 glucanase activity: 400 units/g
  • Lytic activity: >35,000 units/g
  • DNase activity: ND



 
 
Products
Cat. # Product Package Size Price # of Units Select
9005 Westase™ Enzyme 1 g $136.00
 

 



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