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Products >  Molecular_Biology >  Molecular_Biology_Reagents >  General >  Westase

Westase

Westase possesses complex lytic activities for yeast cell walls mainly consisting of beta-1,6 glucanase and beta-1,3 glucanase activity. This enzyme works well for Ascosporogenous yeasts such as Saccharomyces cerevisiae. It can also be used with fission yeasts such as Schizosaccharomyces pombe, which cannot be protoplasted with simple Zymolyase treatment or Basidiosporogenous yeasts.

At-A-Glance Documents

Applications

  • Digestion of yeast cell walls

Source

Streptomyces rochei DB-34. Westase is prepared from liquid culture supernatant of Streptomyces rochei DB-34.

Storage

4°C (dry condition)

Notes

Manufactured by Ozeki Corporation.

Form

Lyophilized powder (containing celite as the excipient)

Definition of Activity

Beta-1,6 glucanase activity: One unit is defined as the amount required to release 1 µmol reducing sugar from 10 mg/mL Pustulan solution in 1 min. at 37°C, pH 6.0.

Lytic activity: One unit is defined as the amount required to cause a 1% decrease in absorbance at 660 nm in 1 min. at 30°C, pH 6.0 when using cell wall fraction of Cryptococcus albidus IFO 0612 as a substrate.

Specific Activity

  • Beta-1,6 glucanase activity: = to 400 units/g
  • Lytic activity: > 35,000 units/g
  • DNase activity: ND



 
 
Products
Cat. # Product Package Size Price # of Units Select
9005 Westase™ Enzyme 1 g $133.00
 

 



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