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Products >  Molecular_Biology >  Molecular_Biology_Reagents >  General >  Proteinase_K

Proteinase K

Proteinase K is a high activity serine protease used in protein digestion. Proteinase K activity is stabilized in the presence of calcium salts and increases with protein denaturants such as SDS or urea. Proteinase K can be used on a wide range of substrates during protein digestion and preferentially degrades ester and peptide bonds next to the C-termini of hydrophobic, sulfuric or aromatic amino acids.

At-A-Glance Documents Images & Data Resources

Features

  • Concentration: 20 mg/mL
  • Form: 20 mM Tris-HCl (pH 7.4), 1 mM CaCl2, 50% glycerol
  • Enzyme Code: 3.4.21.14

Applications

  • DNA, RNA and phage purification
  • Protein digestion
  • Chromosomal DNA preparation for in situ hybridization and fingerprinting, colony/plaque hybridization and pulse-field electrophoresis

Source

Tritirachium album

Purity

Endonuclease, Exonuclease, Nickase and RNase activity not detected by intact gel electrophoresis

Storage

–20°C

Definition of Activity

One unit of enzyme activity is defined as the amount of enzyme required to liberate 1 micromole of Folin positive amino acid in 1 minute at 37°C using denatured bovine hemoglobin as the substrate.

Activity

Greater than or equal to 30 units/mg protein




 
 
Products
Cat. # Product Package Size Price # of Units Select
9033 Proteinase K 5 mL $192.00
 

 



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