- Storing and diluting DNA and RNA
Tris-EDTA Buffer (TE) 10x Powder, pH7.4
10 pouches (for 10 L)
|Final solution (1 L of 10X TE, pH7.4)|
|100 mM Tris-HCl|
|10 mM EDTA|
Store powder at room temperature.
Empty the contents of one pouch in a flask or beaker. Add 300 mL of distilled water or deionized water and stir the solution for a few minutes. Adjust the volume to 1000 mL, and stir until completely dissolved.