- Buffer for agarose and polyacrylamide gel electrophoresis of nucleic acid.
Tris-Acetate-EDTA Buffer (TAE) 50x Powder, pH8.3
1 pouch (for 1 L)
|Final solution (1 L of 50X TAE, pH8.3)|
|2.0 M Tris-acetate|
|0.050 M EDTA|
|RNase/DNase activity: Non-detectable|
Store powder at room temperature.
Transfer the contents of the pouch to a flask or beaker. Add 300 mL of distilled water or deionized water and stir the solution for a few minutes. Adjust the volume to 1000 mL, and stir until completely dissolved.