Applications- Buffer for agarose and polyacrylamide gel electrophoresis of nucleic acid.
ComponentsTris-Acetate-EDTA Buffer (TAE) 50x Powder, pH8.31 pouch (for 1 L) | Final solution (1 L of 50X TAE, pH8.3) | | 2.0 M Tris-acetate | | 0.050 M EDTA | | RNase/DNase activity: Non-detectable |
StorageStore powder at room temperature.
PreparationTransfer the contents of the pouch to a flask or beaker. Add 300 mL of distilled water or deionized water and stir the solution for a few minutes. Adjust the volume to 1000 mL, and stir until completely dissolved.
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