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Products >  Molecular_Biology >  Molecular_Biology_Reagents >  Buffers_and_Powders >  SSC_Powder

Saline Sodium Citrate Buffer (SSC) Powder

Saline Sodium Citrate (SSC) is used as a hybridization buffer or a wash buffer in Southern blotting, in situ hybridization, DNA microarrays, or Northern blotting. These products are powders for preparing SSC buffers; each individual powder-containing pouch yields 1 L of SSC buffer, pH7.0 (2X or 20X).

At-A-Glance Documents Images & Data

Application

  • Hybridization or wash buffer in Southern blotting, in situ hybridization, DNA microarrays, or Northern blotting.

Components

Saline Sodium Citrate Buffer (SSC) 2X Powder, pH7.0 (Cat. # T9171)

5 pouches (for 5 L)

Final Solution from one pouch (1 L of 2X SSC, pH7.0) contains:
0.03 M Sodium citrate
0.3 M NaCl

Saline Sodium Citrate Buffer (SSC) 20X Powder,  pH7.0 (Cat. # T9172)

5 pouches (for 5 L)

Final solution from one pouch (1 L of 20X SSC, pH7.0) contains:
0.3 M Sodium citrate
3.0 M NaCl

Storage

Store powder at room temperature.

Preparation

Empty the contents of one pouch in a flask or beaker. Add 300 mL of distilled water or deionized water and stir the solution briefly. Adjust the volume to 1000 mL, and stir until completely dissolved.




 
 
Products
Cat. # Product Package Size Price # of Units Select
T9171 Saline Sodium Citrate Buffer (SSC) 2X Powder 5 Pouches $60.00
T9172 Saline Sodium Citrate Buffer (SSC) 20X Powder 5 Pouches $123.00
 

 


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