Application- Hybridization or wash buffer in Southern blotting, in situ hybridization, DNA microarrays, or Northern blotting.
ComponentsSaline Sodium Citrate Buffer (SSC) 2X Powder, pH7.0 (Cat. # T9171)5 pouches (for 5 L) | Final Solution from one pouch (1 L of 2X SSC, pH7.0) contains: | | 0.30 M Sodium citrate | | 0.03 M NaCl |
Saline Sodium Citrate Buffer (SSC) 20X Powder, pH7.0 (Cat. # T9172)5 pouches (for 5 L) | Final solution from one pouch (1 L of 20X SSC, pH7.0) contains: | | 3 M Sodium citrate | | 0.3 M NaCl |
StorageStore powder at room temperature.
PreparationEmpty the contents of one pouch in a flask or beaker. Add 300 mL of distilled water or deionized water and stir the solution briefly. Adjust the volume to 1000 mL, and stir until completely dissolved.
|
