- cDNA synthesis (Ribonuclease Inhibitor: 0.5 units/µL reaction)
- In vitro translation (Ribonuclease Inhibitor: 1 unit/µL reaction)
- In vitro transcription using cell-free extract (Ribonuclease Inhibitor: 20 units/µL reaction)
- In vitro transcription with SP6 or T7 RNA Polymerase (Ribonuclease Inhibitor: 1 unit/µL reaction)
- Polysome isolation (Ribonuclease Inhibitor: 1000 units/mL reaction)
2313A: 5000 U
2313B: 5 x 2313A (25000 U)
E. coli containing a plasmid that carries the porcine RNase Inhibitor gene
1. Burton, L.E. & Fucci, N.P. (1982) Int. J. Pept. Protein Res. 19:372-379.
2. Blackburn, P. et al. (1977) J. Biol. Chem. 252:5904-5910.
3. Turner, P.M. et al. (1983) Biochem. Biophys. Res. Comm. 114:1154-1160.
4. Blackburn, P. (1979) J. Biol. Chem. 254:12484-12487.
5. De Martynoff, G. et al. (1980) Biochem. Biophys. Res. Comm. 93:645-653.
6. Scheele, G. & Blackburn, P. (1979) Proc. Natl. Acad. Sci. USA 76:4898-4902.
7. Eichler, D.C. et al. (1981) Biochem. Biophys. Res. Comm. 101:396-403.
One unit is defined as the amount of Recombinant Ribonuclease Inhibitor required to inhibit the activity of 5 ng of RNase A by 50%. Activity is determined as the ability to inhibit hydrolysis of cytidine 2,3'-cyclic monophosphate (cyclic CMP) by RNAse A.