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Products >  Molecular_Biology >  Modifying_Enzymes >  RNase_Inhibitor >  rRibonuclease_Inhibitor

Recombinant Ribonuclease Inhibitor

Takara's Recombinant Ribonuclease Inhibitor is a recombinant protein that acts as a noncompetitive RNase inhibitor of RNase A, B and C. This RNase inhibitor is active over a wide pH range and in the presence of up to 1 mM DTT.

  At-A-Glance   Documents   Images & Data

Applications

  • cDNA synthesis (Ribonuclease Inhibitor: 0.5 units/µL reaction)
  • In vitro translation (Ribonuclease Inhibitor: 1 unit/µL reaction)
  • In vitro transcription using cell-free extract (Ribonuclease Inhibitor: 20 units/µL reaction)
  • In vitro transcription with SP6 or T7 RNA Polymerase (Ribonuclease Inhibitor: 1 unit/µL reaction)
  • Polysome isolation (Ribonuclease Inhibitor: 1000 units/mL reaction)

Components

2313A: 5000 U

2313B: 5 x 2313A (25000 U)

Source

E. coli containing a plasmid that carries the porcine RNase Inhibitor gene

 

Storage

–20°C

Concentration

40 U/µL

References

1. Burton, L.E. & Fucci, N.P. (1982) Int. J. Pept. Protein Res. 19:372-379.

2. Blackburn, P. et al. (1977) J. Biol. Chem. 252:5904-5910.

3. Turner, P.M. et al. (1983) Biochem. Biophys. Res. Comm. 114:1154-1160.

4. Blackburn, P. (1979) J. Biol. Chem. 254:12484-12487.

5. De Martynoff, G. et al. (1980) Biochem. Biophys. Res. Comm. 93:645-653.

6. Scheele, G. & Blackburn, P. (1979) Proc. Natl. Acad. Sci. USA 76:4898-4902.

7. Eichler, D.C. et al. (1981) Biochem. Biophys. Res. Comm. 101:396-403.

Unit Definition

One unit is defined as the amount of Recombinant Ribonuclease Inhibitor required to inhibit the activity of 5 ng of RNase A by 50%. Activity is determined as the ability to inhibit hydrolysis of cytidine 2,3'-cyclic monophosphate (cyclic CMP) by RNAse A.




 
 
Products
Cat. # Product Contents Size Price Units Select
2313A Recombinant RNase Inhibitor 5,000 Units $112.00
2313B Recombinant RNase Inhibitor 25,000 Units $450.00
 

 

 
 

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