Applications - cDNA synthesis (Ribonuclease Inhibitor: 0.5 units/µL reaction)
- In vitro translation (Ribonuclease Inhibitor: 1 unit/µL reaction)
- In vitro transcription using cell-free extract (Ribonuclease Inhibitor: 20 units/µL reaction)
- In vitro transcription with SP6 or T7 RNA Polymerase (Ribonuclease Inhibitor: 1 unit/µL reaction)
- Polysome isolation (Ribonuclease Inhibitor: 1000 units/mL reaction)
Components2313A: 5000 U 2313B: 5 x 2313A (25000 U)
Source Recombinant E. coli (purified via affinity chromatography)
Storage –20°C
Concentration 40 U/µL
References1. Burton, L.E. & Fucci, N.P. (1982) Int. J. Pept. Protein Res. 19:372-379. 2. Blackburn, P. et al. (1977) J. Biol. Chem. 252:5904-5910. 3. Turner, P.M. et al. (1983) Biochem. Biophys. Res. Comm. 114:1154-1160. 4. Blackburn, P. (1979) J. Biol. Chem. 254:12484-12487. 5. De Martynoff, G. et al. (1980) Biochem. Biophys. Res. Comm. 93:645-653. 6. Scheele, G. & Blackburn, P. (1979) Proc. Natl. Acad. Sci. USA 76:4898-4902. 7. Eichler, D.C. et al. (1981) Biochem. Biophys. Res. Comm. 101:396-403.
Unit DefinitionOne unit is defined as the amount of the enzyme required to completely relax 0.5 µg of supercoiled pBR322 DNA in 30 minutes at 37°C in a 50 µL reaction mixture.
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