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Products >  Molecular_Biology >  Modifying_Enzymes >  RNA_Polymerase >  T7_RNA_Polymerase

T7 RNA Polymerase

T7 RNA Polymerase is a DNA-dependent RNA polymerase that exhibits high specificity for the bacteriophage T7 promoter sequence 5'-TAATACGACTCACTATA-3'. The enzyme can incorporate labeled or unlabeled nucleoside triphosphates into an RNA transcript. Large quantities of RNA can be synthesized from a DNA sequence cloned downstream of the T7 promoter due to the high processivity and transcription frequency of T7 RNA Polymerase. The enzyme is supplied in a buffer of 20 mM potassium phosphate (pH 7.9), 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA and 50% glycerol.

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Applications

  • In vitro transcription

Source

Recombinant E. coli

Storage

–20°C

Unit Definition

One unit is defined as the amount of enzyme required to convert 1 nmol of [3H] GMP into acid-insoluble product in 1 hour at 37°C and pH 8.0 using linear DNA as the substrate.

References

1. Davanloo, P. et al. (1984) Proc. Natl. Acad. Sci. USA 81:2035-2039.

2. Chamberlin, M. et al. (1970) Nature 228:227-231.

3. Chamberlin, M. & Ring, J. (1973) J. Biol. Chem. 248:2235-2244.

4. Schenborn, E.T. & Mierendorf Jr., R.C. (1985) Nucleic Acids Res. 13(17):6223-6236.

Buffer

Supplied with 10X Reaction Buffer [400 mM Tris-HCl (pH 8.0), 80 mM MgCl2, 20 mM spermidine and 50 mM DTT].

Concentration

10–50 U/µL




 
 
Products
Cat. # Product Contents Size Price Units Select
2540A T7 RNA Polymerase 5,000 Units $57.00
2540B T7 RNA Polymerase 25,000 Units $225.00
 

 

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