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Products >  Molecular_Biology >  Modifying_Enzymes >  RNA_Polymerase >  T7_RNA_Polymerase

T7 RNA Polymerase

T7 RNA Polymerase is a DNA-dependent RNA polymerase that exhibits a high specificity for bacteriophage T7 promoter sequences. The enzyme can incorporate labeled or unlabeled nucleotide triphosphates into an RNA transcript. Large quantities of RNA can be synthesized from a DNA sequence cloned downstream from a T7 promoter. T7 RNA Polymerase is supplied in 20 mM potassium phosphate (pH 7.9), 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA and 50% glycerol.
At-A-Glance Documents

Applications

  • Synthesis of RNA Transcripts

Source

Recombinant E. coli

Storage

–20°C

Unit Definition

One unit is defined as the amount of enzyme that converts 1 nmol of [3H] GMP using a linear DNA template into acid-insoluble material in 1 hour at 37°C and pH 8.0.

Buffer

Supplied with 10X Reaction Buffer [400 mM Tris-HCl (pH 8.0), 80 mM MgCl2 and 20 mM spermidine], and 50 mM DTT.

Concentration

10–50 U/µL


 
 
Products
Cat. # Product Package Size Price # of Units Select
2540A T7 RNA Polymerase 5,000 Units $56.00
2540B T7 RNA Polymerase 25,000 Units $221.00
 

 

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