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Products >  Molecular_Biology >  Modifying_Enzymes >  RNA_Polymerase >  SP6_RNA_Polymerase

SP6 RNA Polymerase

SP6 RNA Polymerase is a DNA-dependent RNA polymerase that exhibits high specificity for the bacteriophage SP6 promoter sequence 5'-TAATCCACTGTGATAT-3'. The enzyme can incorporate labeled or unlabeled ribonucleoside triphosphates into an RNA transcript. Large quantities of RNA can be synthesized from a DNA sequence cloned downstream of the SP6 promoter due to the high processivity of SP6 RNA Polymerase. The enzyme is supplied in a buffer consisting of 10 mM potassium phosphate (pH 7.9), 150 mM NaCl, 1 mM DTT, 0.1 mM EDTA and 50% glycerol.

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Applications

  • In vitro transcription 

Source

Recombinant E. coli

Storage

–20°C

Unit Definition

One unit is defined as the amount of enzyme required to incorporate 1 nmol of [3H] GMP into acid-insoluble product in 1 hour at 37°C and pH 7.5 using a linear DNA template.

References

1. Kotani, H. et al. (1987) Nucleic Acids Res. 15:2653-2664.

2. Butler, E.T. & Chamberlin, M.J. (1982) J. Biol. Chem. 257:5772-5778.

3. Melton, D.A. et al. (1984) Nucleic Acids Res. 12:7035-7056.

4. Schenborn, Jr., E.T. & Mierendorf, R.C. (1985) Nucleic Acids Res. 13:6223-6236.

5. Green, M.R. et al. (1983) Cell 32:681-694.

6. Polletier, J. & Sonenberg, N. (1985) Cell 40:515-526.

7. Sagawa, H. et al. (1996) Gene 168:37-41.

Buffer

Supplied with 10X Reaction Buffer [400 mM Tris-HCl (pH 7.5), 60 mM MgCl2, 20 mM spermidine, 100 mM DTT, 0.1% BSA].

Concentration

10–50 U/µL




 
 
Products
Cat. # Product Contents Size Price Units Select
2520A SP6 RNA Polymerase 3,000 Units $225.00
2520B SP6 RNA Polymerase 15,000 Units $900.00
 

 

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