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Products >  Molecular_Biology >  Modifying_Enzymes >  Phosphatases >  Alkaline_Phosphatase_Shrimp

Shrimp Alkaline Phosphatase (SAP)

Shrimp Alkaline Phosphatase (SAP) nonspecifically catalyzes the dephosphorylation of 5'-termini DNA and RNA phosphomonoesters but does not degrade diphosphate or triphosphate linkages. The enzyme acts on 5' protruding, recessive and blunt 5'-termini. Unlike Calf Intestinal Alkaline Phosphatase, SAP is irreversibly heat inactivated at 65°C for 15 minutes, allowing for streamlining of cloning experiments.

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Applications

  • Dephosphorylation of DNA 5'-termini
  • Reduction of vector re-ligation and background

Source

Shrimp (Pandalus borealis)

Storage

–20°C

Buffer

Supplied with 1ml 10X SAP Buffer [500 mM Tris-HCl (pH 9.0), 50 mM MgCl2].

References

  1. Rangnar, L. et al. (1991) Comp. Biochem. Physiol. 99B(4):755-761.

Concentration

1 U/µL

Unit Definition

One unit is defined as the amount of enzyme required to produce 1 µmol/min of p-nitrophenol at 37°C (pH 9.6) using p-nitrophenylphosphate as the substrate.




 
 
Products
Cat. # Product Contents Size Price Units Select
2660A Alkaline Phosphatase (Shrimp) 300 Units $150.00
2660B Alkaline Phosphatase (Shrimp) 1,500 Units $603.00
 

 

 
 

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