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Products >  Molecular_Biology >  Modifying_Enzymes >  Phosphatases >  Alkaline_Phosphatase_Shrimp

Alkaline Phosphatase, Shrimp

Shrimp Alkaline Phosphatase (SAP) nonspecifically catalyzes the dephosphorylation of most phosphate monoesters, but it does not degrade diphosphate or triphosphate linkages. It is irreversibly inactivated by heat denaturation at 65°C for 15 minutes.

At-A-Glance Documents Resources

Applications

  • Dephosphorylation of 5'-terminal phosphate groups from DNA

Source

Shrimp (Pandalus borealis)

Storage

–20°C

Buffer

Supplied with 1ml 10X SAP Buffer [500 mM Tris-HCl (pH 9.0), 50 mM MgCl2].

References

  1. Rangnar, L. et al. (1991) Comp. Biochem. Physiol. 99B(4): 755-761.

Concentration

1 U/µL

Unit Definition

One unit is the amount of enzyme that produces 1 µmol/min of p-nitrophenol per minute at 37°C and pH 9.6 using p-nitrophenylphosphate as the substrate.




 
 
Products
Cat. # Product Package Size Price # of Units Select
2660A Alkaline Phosphatase (Shrimp) 300 Units $147.00
2660B Alkaline Phosphatase (Shrimp) 1,500 Units $591.00
 

 



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