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Products >  Molecular_Biology >  Modifying_Enzymes >  Phosphatases >  Alkaline_Phosphatase_E_coli

Bacterial Alkaline Phosphatase (E. coli)

Bacterial Alkaline Phosphatase (E.coli) nonspecifically catalyzes the removal of most phosphomonoester bonds from the 5' and 3' termini of DNA and RNA without degrading diphosphate or triphosphate linkages. The enzyme is purified from an E. coli strain that does not harbor RNase activity. Bacterial Alkaline Phosphatase (E. coli) is supplied in a buffer of 50 mM Tris-HCl (pH 8.0), 100 mM KCl, 1 mM MgCl2 and 50% glycerol.

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Applications

  • Phosphate group removal to reduce self-ligation and vector background. 
  • Dephosphorylation of DNA 5' termini DNA prior to kinase labeling reactions

 

Source

E. coli strain C75

Storage

–20°C

Notes

Bacterial Alkaline Phosphatase (E.coli) is extremely stable and is not completely heat inactivated; therefore, reaction products need to be twice purified via phenol-chloroform extraction followed by ethanol precipitation.

Buffer

Supplied with 10X Reaction Buffer [500 mM Tris-HCl (pH 9.0), 10 mM MgCl2]

References

  1. Reid, T.W. & Wilson, I.B. (1971) The Enzymes 3:373-416.
  2. Takanami, M. (1967) J. Mol. Biol. 23:135-148.

Unit Definition

One unit is defined as the amount of enzyme required to generate 1 µmol/min of p-nitrophenol from p-nitrophenylphosphate at 25°C and pH 8.0.

Concentration

0.3–0.6 U/µL




 
 
Products
Cat. # Product Contents Size Price Units Select
2120A Alkaline Phosphatase (E coli C75) 50 Units $80.00
2120B Alkaline Phosphatase (E coli C75) 250 Units $315.00
 

 

 
 

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