Applications - Removing single phosphate groups from 5'-ends of linear vectors to prevent recircularization during cloning
- Dephosphorylation of DNA prior to kinase labeling reactions
Source E. coli strain C75
Storage –20°C
Buffer Supplied with 10X Reaction Buffer [500 mM Tris-HCl (pH 9.0), 10 mM MgCl2].
References - Reid, T.W. and Wilson, I.B. (1971) in The Enzymes 3:373-416.
- Takanami, M. (1967) J. Mol. Biol. 23:135-48.
Unit Definition One unit is defined as the amount of enzyme that generates 1 µmol/min of p-nitrophenol from p-nitrophenylphosphate at 25°C and pH 8.0.
Concentration 0.3–0.6 U/µL
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