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Products >  Molecular_Biology >  Modifying_Enzymes >  Phosphatases >  Alkaline_Phosphatase_Calf_Intestinal

Calf Intestinal Alkaline Phosphatase (CIAP)

Calf Intestinal Alkaline Phosphatase (CIAP) catalyzes the hydrolysis of 5'-phosphate termini from DNA, RNA, dNTPs and rNTPs. CIAP is supplied in a buffer of 10 mM Tris-HCl (pH 8.0), 50 mM KCl, 1 mM MgCl2, 0.1 mM ZnCl2 and 50% glycerol.

At-A-Glance Documents Images & Data Resources

Applications

  • Reduction of linear vector self-ligation and vector background during cloning
  • Dephosphorylation of DNA prior to kinase labeling reactions
  • Template preparation prior to 5'-end labeling
  • Protein dephosphorylation

Source

Yeast

Storage

–20°C

Notes

Calf intestinal alkaline phosphatase (CIAP) cannot be completely heat inactivated and therefore it is recommended that the DNA product be purified by gel or spin column purification or phenol-chloroform extraction. 

Buffer

Supplied with 10X Reaction Buffer [500 mM Tris-HCl (pH 9.0), 10 mM MgCl2].

References

  1. Sambrook, J. et al. (1989) Molecular Cloning: A Laboratory Manual, pp 5.72, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.

Unit Definition

One unit is defined as the amount of enzyme that generates 1 µmol/min of p-nitrophenol from p-nitrophenylphosphate at 37°C and pH 9.8.

Concentration

10–30 U/µL




 
 
Products
Cat. # Product Package Size Price # of Units Select
2250A Alkaline Phosphatase (Calf Intestine) 1,000 Units $80.00
2250B Alkaline Phosphatase (Calf Intestine) 5,000 Units $315.00
 

 


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