Applications - Removing phosphate groups from 5'-ends of linear vectors to prevent recircularization during cloning
- Dephosphorylation of DNA prior to kinase labeling reactions
Source Yeast
Storage –20°C
Buffer Supplied with 10X Reaction Buffer [500 mM Tris-HCl (pH 9.0), 10 mM MgCl2].
References - Sambrook, J. et al. (1989) Molecular Cloning: A Laboratory Manual, pp 5.72, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
Unit Definition One unit is defined as the amount of enzyme that generates 1 µmol/min of p-nitrophenol from p-nitrophenylphosphate at 37°C and pH 9.8.
Concentration 10–30 U/µL
|
