- Excellent thermostability (i.e., can be stored at room temperature)
- Efficiently digests native chitin
- Can be used on its own to prepare protoplasts from filamentous fungi
- Cell wall digestion of filamentous fungi
Corynebacterium sp. OZ-21
Lyophilized preparation is stable for at least 1 year at 4°C.
Enzyme activities found in Yatalase
|Enzyme activity||Activity |
|Cell-well lysing activity||10,000|
Yields of Protoplasts
|Species||Yield of protoplasts |
(× 106/g wet cells)
- Optimum pH: 7.2
- Optimum temperature for enzyme activity: 37°C
Lyophilized powder (containing lactose)
1. Tsuchiya, K. et al. (2003) Food Sci. Tech. Res. 9(3):283-287.
2. Iwashita, K. et al. (1998) Biosci. Biotechnol. Biochem. 62(10):1938-1946.
Chitinase activity: One unit is defined as the amount of enzyme required to release 1 µmol of N-acetylglucosamine from chitin in 1 minute.
Chitobiase activity: One unit is defined as the amount of enzyme required to release 1 µmol of p-nitrophenol from p-nitrophenyl-N-acetyl-beta-D-glucosaminide in 1 minute.
Lytic activity: One unit is defined as the amount of enzyme required to decrease absorbance by 1% in 1 hour.
- Chitinase activity: ~50 U/g powder
- Chitobiase activity: ~500 U/g powder
- Lytic activity: ~10,000 U/g powder