Features - Excellent thermostability (i.e., can be stored at room temperature)
- Efficiently digests native chitin
- Can be used on its own to prepare protoplasts from filamentous fungi
Applications - Cell wall digestion of filamentous fungi
SourceCorynebacterium sp. OZ-21
StorageLyophilized preparation is stable for at least 1 year at 4°C.
NotesEnzyme activities found in Yatalase| Enzyme activity | Activity
(U/g power) |
| | Chitinase | 50 | | Chitobiase | 500 | | Chitosanase | 19 | | beta-1,3-glucanase | 300 | | Protease | 31 | | Cell-well lysing activity | 10,000 |
Yields of Protoplasts | Species | Yield of protoplasts
(× 106/g wet cells) |
| | Aspergillus oryzae | 70 | | Aspergillus kawachi | 2 | | Aspergillus terreus | 21 | | Penicillium citrinum | 20 | | Penicillium lanosum | 50 | | Trichoderma koningii | 100 | | Monascus sp. | 14 | | Mucor hiemalis | 8 | | Rhizopus nigricans | 21 | | Pleurotus ostreatus | 11 | | Coprinus cinereus | 20 | | Lentinus edodes | 2 |
Properties:- Optimum pH: 7.2
- Optimum temperature for enzyme activity: 37°C
FormLyophilized powder (containing lactose)
References1. Tsuchiya, K. et al. (2003) Food Sci. Tech. Res. 9(3):283-287. 2. Iwashita, K. et al. (1998) Biosci. Biotechnol. Biochem. 62(10):1938-1946.
Unit DefinitionChitinase activity: One unit is defined as the amount of enzyme required to release 1 µmol of N-acetylglucosamine from chitin in 1 minute. Chitobiase activity: One unit is defined as the amount of enzyme required to release 1 µmol of p-nitrophenol from p-nitrophenyl-N-acetyl-beta-D-glucosaminide in 1 minute. Lytic activity: One unit is defined as the amount of enzyme required to decrease absorbance by 1% in 1 hour.
Specific Activities- Chitinase activity: ~50 U/g powder
- Chitobiase activity: ~500 U/g powder
- Lytic activity: ~10,000 U/g powder
|
