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Products >  Molecular_Biology >  Modifying_Enzymes >  Nucleases >  Yatalase

Yatalase

Yatalase is commonly used for filamentous fungi cell wall digestion, enabling protoplast preparation. The product is prepared from cell culture supernatants of Corynebacterium sp. OZ-21 and consists mainly of chitinase, chitobiase and beta-1,3-glucanase.

This product is manufactured by Ozeki Corporation.

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Features

  • Excellent thermostability (i.e., can be stored at room temperature)
  • Efficiently digests native chitin
  • Can be used on its own to prepare protoplasts from filamentous fungi

Applications

  • Cell wall digestion of filamentous fungi

Source

Corynebacterium sp. OZ-21

Storage

Lyophilized preparation is stable for at least 1 year at 4°C.

Notes

Enzyme activities found in Yatalase

Enzyme activityActivity
(U/g power)

Chitinase50
Chitobiase500
Chitosanase*19
beta-1,3-glucanase*300
Protease*31
Cell-well lysing activity10,000

*representative value, not a standard value

Yields of Protoplasts

SpeciesYield of protoplasts
(× 106/g wet cells)

Aspergillus oryzae70
Aspergillus kawachi2
Aspergillus terreus21
Penicillium citrinum20
Penicillium lanosum50
Trichoderma koningii100
Monascus sp.14
Mucor hiemalis8
Rhizopus nigricans21
Pleurotus ostreatus11
Coprinus cinereus20
Lentinus edodes2

Properties:

  • Optimum pH: 7.2
  • Optimum temperature for enzyme activity: 37°C

Form

Lyophilized powder (containing lactose)

References

1. Tsuchiya, K. et al. (2003) Food Sci. Tech. Res. 9(3):283-287.

2. Iwashita, K. et al. (1998) Biosci. Biotechnol. Biochem. 62(10):1938-1946.

Unit Definition

Chitinase activity: One unit is defined as the amount of enzyme required to release 1 µmol of N-acetylglucosamine from chitin in 1 minute.

Chitobiase activity: One unit is defined as the amount of enzyme required to release 1 µmol of p-nitrophenol from p-nitrophenyl-N-acetyl-beta-D-glucosaminide in 1 minute.

Lytic activity: One unit is defined as the amount of enzyme required to decrease absorbance by 1% in 1 hour.

Specific Activities

  • Chitinase activity: ~50 U/g powder
  • Chitobiase activity: ~500 U/g powder
  • Lytic activity: ~10,000 U/g powder



 
 
Products
Cat. # Product Contents Size Price Units Select
T017 Yatalase™ Enzyme 2 g $225.00
 

 

 
 

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