Features - Excellent thermostability and can be stored at room temperature
- Efficiently digests native Chitin
- Can be used alone to prepare protoplasts from filamentous fungi
Applications - Lyse Cell Walls of Filamentous Fungi
Source Corynebacterium sp. OZ-21
Storage Lyophilized preparation is stable for at least 1 year at 4°C.
Notes Enzyme activities found in Yatalase | Enzyme activity | Activity
(units/g power) |
| | Chitinase | 50 | | Chitobiase | 500 | | Chitosanase | 19 | | beta-1,3-glucanase | 300 | | Protease | 31 | | Cell-well lysing activity | 10,000 | Yields of Protoplasts | Species | Yield of protoplasts
(× 106/g-wet cells) |
| | Aspergillus oryzae | 70 | | Aspergillus kawachi | 2 | | Aspergillus terreus | 21 | | Penicillium citrinum | 20 | | Penicillium lanosum | 50 | | Trichoderma koningii | 100 | | Monascus sp. | 14 | | Mucor hiemalis | 8 | | Rhizopus nigricans | 21 | | Pleurotus ostreatus | 11 | | Coprinus cinereus | 20 | | Lentinus edodes | 2 |
Properties: - Optimum pH: pH 7.2
- Optimum temperature for enzyme activity: 37°C
Form Lyophilized powder (containing lactose)
Unit Definition Chitinase activity: One unit of chitinase activity is determined as the amount required to release 1 µmol of N-acetylglucosamine from chitin in 1 minute. Chitobiase activity: One unit of chitobiase activity is defined as the amount of enzyme required to release 1 µmol of p-nitrophenol from p-nitrophenyl-N-acetyl-beta-D-glucosaminide in 1 minute. Lytic activity: One unit of enzyme activity is defined as the amount required to cause a 1% decrease in absorbance in 1 hour.
Specific Activities - Chitinase activity: Approximately 50 units/g powder
- Chitobiase activity: Approximately 500 units/g powder
- Lytic activity against cell walls: Approximately 10,000 units/g powder
|
