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Products >  Molecular_Biology >  Modifying_Enzymes >  Nucleases >  S1_Nuclease

S1 Nuclease

S1 Nuclease is an endonuclease that specifically degrades single-stranded nucleic acids, including the single-stranded regions of duplex DNA, RNA or DNA/RNA. S1 Nuclease preferentially targets DNA over RNA. This endonuclease can also introduce single-stranded nicks and breaks in duplex DNA, RNA and DNA/RNA.

S1 Nuclease is supplied in a buffer of 10 mM sodium acetate (pH 4.6), 150 mM NaCl, 0.05 mM ZnSO4 and 50% glycerol.

At-A-Glance Documents Images & Data

Applications

  • Nuclease protection assays
  • Double-stranded DNA end blunting
  • RNA transcript mapping
  • cDNA hairpin removal 

Source

Aspergillus oryzae

Storage

–20°C

Buffer

Supplied with 10X Reaction Buffer [300 mM sodium acetate (pH 4.6), 2.8 M NaCl and 10 mM ZnSO4].

References

  1. Sambrook, J. et al. (1989) Molecular Cloning: A Laboratory Manual, pp. 5.78-5.79, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
  2. Berk, A.J. & Sharp, P.A. (1978) Proc. Natl. Acad. Sci. USA 75:1274-1278.
  3. Ando, T. (1966) Biochim. Biophys. Acta 114:158-168.
  4. Wiegand, R.C. et al. (1975) J. Biol. Chem. 250:8848-8855.

Unit Definition

One unit is defined as the amount of enzyme required to convert 1 µg of heat-denatured calf thymus DNA into acid-soluble product in 1 minute at 37°C and pH 4.6.

Concentration

100–200 U/µL




 
 
Products
Cat. # Product Package Size Price # of Units Select
2410A S1 Nuclease 20,000 Units $67.00
2410B S1 Nuclease 100,000 Units $270.00
 

 


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