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Products >  Molecular_Biology >  Modifying_Enzymes >  Nucleases >  S1_Nuclease

S1 Nuclease

S1 Nuclease specifically degrades single-stranded nucleic acids, including single-stranded regions of duplex DNA or RNA. S1 Nuclease is supplied in 10 mM sodium acetate (pH 4.6), 150 mM NaCl, 0.05 mM ZnSO4 and 50% glycerol.
At-A-Glance Documents

Applications

  • Creating blunt ends from protruding ends in double-stranded DNA fragments
  • Digestion of single-stranded regions in double-stranded DNA
  • Mapping RNA transcripts

Source

Aspergillus oryzae

Storage

–20°C

Buffer

Supplied with 10X Reaction Buffer [300 mM sodium acetate (pH 4.6), 2.8 M NaCl and 10 mM ZnSO4].

References

  1. Sambrook, J. et al. (1989) Molecular Cloning: A Laboratory Manual, pp. 5.78-5.79, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
  2. Berk, A.J. and Sharp, P.A. (1978) Proc. Natl. Acad. Sci. USA 75:1274-78.

Unit Definition

One unit is defined as the amount of enzyme that converts 1 µg of heat-denatured calf thymus DNA into acid-soluble products in 1 minute at 37°C and pH 4.6.

Concentration

100–200 U/µL


 
 
Products
Cat. # Product Package Size Price # of Units Select
2410A S1 Nuclease 20,000 Units $66.00
2410B S1 Nuclease 100,000 Units $265.00
 

 


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