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Products >  Molecular_Biology >  Modifying_Enzymes >  Nucleases >  Ribonuclease_H-RNase_H

Ribonuclease H (RNase H)

Ribonuclease H (RNase H) is an RNA-specific endonuclease that hydrolyzes single-stranded RNA via a magnesium or manganese cofactor. As such, RNase H is often used to remove the RNA template following reverse transcription or cDNA generation. Ribonuclease H (RNase H) is supplied in a buffer of 25 mM Tris-HCl (pH 7.5), 30 mM NaCl, 0.5 mM EDTA, 1 mM DTT and 50% glycerol.
At-A-Glance Documents Images & Data

Applications

  • Removal of RNA template following second-strand cDNA synthesis
  • Removal of mRNA poly(A) sequence
  • Reverse transcription

Source

Recombinant E. coli

Storage

–20°C

Unit Definition

One unit is defined as the amount of enzyme required to produce 1 nmol of acid-soluble product in 20 minutes at 30°C and pH 7.7 using poly(rA)-poly(dT) as a substrate.

References

  1. Vournakis, J.N. et al. (1975) Proc. Natl. Acad. Sci. USA 72:2959-63.
  2. Schultz, S.J. & Champoux, J.J. (2008) Virus Res. 134(1–2):86–103.

Concentration

20–60 U/µL


 
 
Products
Cat. # Product Package Size Price # of Units Select
2150A Ribonuclease H 1,000 Units $80.00
2150B Ribonuclease H 5,000 Units $315.00
 

 


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