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Products >  Molecular_Biology >  Modifying_Enzymes >  Nucleases >  Recombinant_DNase_I-RNase-free

Recombinant DNase I (RNase-free)

Recombinant DNase I (RNase-free) is an endonuclease that nonspecifically catalyzes the degradation of both single- and double-stranded DNA and DNA-RNA hybrids, producing 5'-phosphate and 3' hydroxyl termini-containing oligonucleotides of varied lengths. The protease activity of DNase I Endonuclease has been eliminated; therefore, this enzyme is stable at its optimal (neutral) pH range and is suitable for RNA preparation at neutral pH. Recombinant DNase I (RNase-free) is heat inactivated at 80°C for 10 minutes.
At-A-Glance Documents Images & Data

Applications

  • Template DNA digestion following in vitro transcription
  • Genomic DNA digestion prior to RT-PCR
  • Nick translation with DNA Polymerase
  • Shotgun DNA library construction
  • Footprinting analysis

Components

Supplied Buffer (10X)

Tris-HCl, pH7.5400 mM
MgCl280 mM
DTT50 mM

Source

Recombinant non-animal host containing bovine pancrease DNase I-encoding plasmid

Storage

–20°C

Unit Definition

One unit is defined as the amount of enzyme required to increase 260 nm absorbance by 0.001 per minute at 25°C (pH 5.0) using calf thymus DNA as the substrate (Kunitz unit).

References

  1. Kunitz, M. (1950) J. Gen. Physiol., 33:349-377.
  2. Rigby, P. W. J. et al. (1977) J. Mol. Biol. 113:237-251.
  3. Anderson, S. (1981) Nucleic Acids Res. 9:3015-3027.
  4. Galas, D. J. & Schmitz, A. (1978) Nucleic Acids Res. 5:3157-3170.
  5. Green, M. R. et al. (1983) Cell 32:681-694.

Concentration

5 U/µL


 
 
Products
Cat. # Product Package Size Price # of Units Select
2270A Recombinant DNase I (RNase-free) 1,000 Units $84.00
2270B Recombinant DNase I (RNase-free) 5,000 Units $335.00
 

 


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