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Products >  Molecular_Biology >  Modifying_Enzymes >  Nucleases >  Recombinant_DNase_I-RNase-free

Recombinant DNase I (RNase-free)

Recombinant DNase I (RNase-free) is an endonuclease that catalyzes, to the same extent, the degradation of both single- and double-stranded DNA randomly, and produces 5'-P terminal oligonucleotides. Protease activity has been eliminated; therefore, this enzyme is stable around its optimum neutral pH range and is suitable for RNA preparation at neutral pH.
At-A-Glance Documents

Applications

  • Digestion of template DNA after in vitro transcription with T7 or SP6 RNA Polymerase
  • Digestion of genomic DNA before RT-PCR
  • Nick translation with DNA Polymerase
  • Construction of a shotgun DNA library in the presence of Mn2+
  • Foot-printing analysis of the interaction between DNA and protein

Components

Supplied Buffer (10X)

Tris-HCl, pH7.5 400 mM
MgCl2 80 mM
DTT 50 mM

Source

Expressed in non-animal host carrying plasmid encodes the gene of bovine pancreas DNase I.

Storage

–20°C

Unit Definition

One unit is the amount of enzyme that increases the absorbance at 260 nm by 0.001 per minute at 25°C, pH 5.0, with calf thymus DNA as the substrate (Kunitz unit).

References

  1. Kunitz, M. (1950) J. Gen. Physiol., 33, 349-377.
  2. Rigby, P. W. J., Dieckmann, M., Rhodes, C. and Berg, P. (1977) J. Mol. Biol., 113, 237-251.
  3. Anderson, S. (1981) Nucleic Acids Res., 9, 3015-3027.
  4. Galas, D. J. and Schmitz, A. (1978) Nucleic Acids Res., 5, 3157-3170.
  5. Green, M. R., Maniatis, T. and Melton, D. A. (1983 ) Cell, 32, 681-694.

Concentration

5 U/µL


 
 
Products
Cat. # Product Package Size Price # of Units Select
2270A Recombinant DNase I (RNase-free) 1,000 Units $82.00
2270B Recombinant DNase I (RNase-free) 5,000 Units $328.00
 

 


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