Takara
login | register | order now | view cart | feedback
Products
Molecular Biology


Products >  Molecular_Biology >  Modifying_Enzymes >  Nucleases >  Mung_Bean_Nuclease

Mung Bean Nuclease

Mung Bean Nuclease acts as a preferential DNA and RNA endonuclease, hydrolyzing single-stranded DNA and RNA into 5'-phosphate and 3'-hydroxyl-containing products. If used at very high concentrations, Mung Bean Nuclease is also capable of degrading double-stranded DNA, RNA or DNA/RNA fragments from both ends. Due to its single-stranded DNA and RNA endonuclease activity, Mung Bean Nuclease is often used during transcript mapping, duplex or hybrid DNA and RNA blunt ending, and cDNA preparation. 

Mung Bean Nuclease is supplied in a buffer of 10 mM Tris-HCl (pH 7.5), 0.1 mM zinc acetate and 50% glycerol.

At-A-Glance Documents Images & Data

Applications

  • Double-stranded DNA, RNA or DNA/RNA blunt ending
  • cDNA hairpin loop cleavage

Source

Mung bean sprouts

Storage

–20°C

Buffer

Supplied with 10X Reaction Buffer [300 mM sodium acetate (pH 5.0), 1000 mM NaCl, 10 mM zinc acetate and 50% glycerol].

References

  1. Sheflin, L.G. & Kowalski, D. (1985) Nucleic Acids Res. 13:6137-6154.
  2. Kowalski, D. et al. (1976) Biochemistry 15:4457-4463.
  3. Kroeker, W.D. et al. (1976) Biochemistry 15:4463-4467.
  4. Mathis, D.J. et al. (1981) Proc. Natl. Acad. Sci. USA 78:7383-7387.

Unit Definition

One unit is defined as the amount of enzyme required to convert 1 µg of heat-denatured calf thymus DNA into acid-soluble product in 1 minute at 37°C and pH 5.0.

Concentration

10–50 U/µL




 
 
Products
Cat. # Product Package Size Price # of Units Select
2420A Mung Bean Nuclease 2,000 Units $67.00
2420B Mung Bean Nuclease 10,000 Units $270.00
 

 



Clontech is a Takara Bio Company © 2014 Privacy Policy | Terms & Conditions