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Products >  Molecular_Biology >  Modifying_Enzymes >  Nucleases >  Mung_Bean_Nuclease

Mung Bean Nuclease

Mung Bean Nuclease digests single-stranded nucleic acids (both DNA and RNA). If used at very high concentrations, it is capable of degrading double-stranded DNA fragments. Mung Bean Nuclease is supplied in 10 mM Tris-HCl (pH 7.5), 0.1 mM zinc acetate and 50% glycerol.
At-A-Glance Documents

Applications

  • Creating blunt ends from protruding ends in double-stranded DNA fragments
  • Digestion of single-stranded regions in duplex DNA or RNA-DNA hybrids

Source

Mung bean sprouts

Storage

–20°C

Buffer

Supplied with 10X Reaction Buffer [300 mM sodium acetate (pH 5.0), 1000 mM NaCl, 10 mM zinc acetate and 50% glycerol].

References

  1. Sheflin, L.G. and Kowalski, D. (1985) Nucleic Acids Res. 13:6137-54.
  2. Kowalski, D. et al. (1976) Biochemistry 15:4457-63.
  3. Kroeker, W.D. et al. (1976) Biochemistry 15:4463-7.

Unit Definition

One unit is defined as the amount of enzyme that converts 1 µg of heat-denatured calf thymus DNA into acid-soluble products in 1 minute at 37°C and pH 5.0.

Concentration

10–50 U/µL


 
 
Products
Cat. # Product Package Size Price # of Units Select
2420A Mung Bean Nuclease 2,000 Units $66.00
2420B Mung Bean Nuclease 10,000 Units $265.00
 

 


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