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Products >  Molecular_Biology >  Modifying_Enzymes >  Nucleases >  Micrococcal_Nuclease

Micrococcal Nuclease

Micrococcal Nuclease is an endonuclease that preferentially digests single-stranded DNA or RNA especially at AT or AU-rich regions. The enzyme will also digest double-stranded DNA or RNA, making it an essential component of chromatin immunoprecipitation (ChIP) assays. Micrococcal nuclease digests 5'-phosphodiester bonds of DNA and RNA, yielding 3'-phosphate mononucleotides and oligonucleotides. This enzyme requires Ca2+ as a cofactor for its activity and is completely inactivitated by EDTA or EGTA.

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Applications

  • Digestion of nucleotides included in crude cell extract
  • Digestion of chromatin for nucleosome preparation
  • Chromatin immunoprecipitation (ChIP)

Supplied Buffer

10X Micrococcal Nuclease [200 mM Tris-HCl (pH 8.0), 50 mM NaCl, 25 mM CaCl2]

Source

Staphylococcus aureus

Purity

Nuclease activity is not detected after incubation of 1 µg lambda DNA with 50 units of this enzyme for 10 minutes at 37°C in the reaction enzyme buffer (1 x H buffer), as judged from the agarose gel electrophoresis pattern. This enzyme is more than 95% homogenous as judged by SDS-PAGE.

Storage

–20°C

Concentration

15–30 units/µL

References

1. Lefevre, P. & Bonifer, C. (2006) Methods Mol. Biol. 325:315-325.

2. Heins, J.N. et al. (1966) Procedures Nucl. Acids Res. 1:79.

3. Pelham, H.R. & Jackson, R.J. (1976) Eur. J. Biochem. 67:247-256.

4. Telford, D.J. & Stewart, B.W. (1989) Int. J. Biochem. 21:127-137.

Unit Definition

One unit is defined as the amount of enzyme required to produce 1 OD260 of acid-soluble product in 30 minutes at 37°C and pH 8.0 using heat-denatured calf thymus DNA as the substrate.

Form

50 mM Tris-HCl (pH 8.0), 10 mM 2-mercaptoethanol, 50 mM NaCl, 50% glycerol




 
 
Products
Cat. # Product Contents Size Price Units Select
2910A Micrococcal Nuclease 15,000 Units $141.00
 

 

 
 

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