Applications - Digestion of nucleotides included in crude cell extract
- Digestion of chromatin for nucleosome preparation
Supplied Buffer 10X Micrococcal Nuclease [200 mM Tris-HCl (pH 8.0), 50 mM NaCl, 25 mM CaCl2]
Source Staphylococcus aureus
Purity Nuclease activity is not detected after incubation of 1 µg lambda DNA with 50 units of this enzyme for 10 minutes at 37°C in the reaction enzyme buffer (1 x H buffer), as judged from the agarose gel electrophoresis pattern. This enzyme is more than 95% homogenous as judged by SDS-PAGE.
Storage –20°C
Concentration 15–30 units/µL
Unit Definition One unit is the amount of enzyme that produces 1 OD260 of acid-soluble products in 30 minutes at 37°C and pH 8.0, with heat-denatured calf thymus DNA as the substrate.
Form 50 mM Tris-HCl (pH 8.0), 10 mM 2-mercaptoethanol, 50 mM NaCl, 50% glycerol
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