Applications - Preparation of partially single-stranded DNA for sequencing
- Producing nested deletions in Double-stranded DNA
- Analyzing DNA-protein interactions
Source Recombinant E. coli
Storage –20°C (when stored for more than 6 months, it should be kept frozen at –80°C)
Buffer Supplied with 10X Reaction Buffer [500 mM Tris-HCl (pH 8.0), 50 mM MgCl2 and 100 mM DTT.]
References - Wu, C. (1985) Nature 317:84-7.
- Guo, L.H. and Wu, R. (1983) Methods Enzymol. 100:60-96.
Unit Definition One unit is defined as the amount of enzyme that produces 1 nmol of acid-soluble products in 30 minutes at 37°C and pH 8.0, with restriction-digested calf thymus DNA as a substrate.
Concentration 100–300 U/µL
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