Applications- Single-stranded DNA degradation
- Removal of ssDNA fragments in a reaction mixture
- PCR primer digestion post-reaction
SourceRecombinant E. coli JM109 encoding E. coli Exonuclease I
Storage–20°C
NotesExonuclease I should not be used for DNA end blunting; short DNA ends are not an appropriate substrate for Exonuclease I. For DNA end blunting, use DNA Polymerase I, Klenow Fragment (2140A, 2140AK, 2140B, 2140BK) or Mung Bean Nuclease (2420A, 2420B) instead.
Unit Definition One unit is defined as the amount of enzyme required to produce 10 nmol of acid-soluble product in 30 minutes at 37°C and pH 9.5 using heat-denaturated calf thymus DNA as the substrate.
Concentration 5 U/µL
BufferSupplied with 1 mL 10X Exonuclease I Buffer [670 mM Glycine-KOH, pH 9.5, 10 mM DTT, 67 mM MgCl2].
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