Applications - Removal of ssDNA fragments in a reaction mixture
- Digestion of remaining primers in a PCR reaction mixture after cycling
Source Escherichia coli JM109 carrying plasmids encoding the gene for E. coli Exonuclease I
Storage –20°C
Unit Definition One unit is the amount of enzyme that produces 10 nmol of acid-soluble products in 30 minutes at 37°C and pH 9.5 using heat-denaturated calf thymus DNA as the substrate.
Concentration 5 U/µL
Buffer Supplied with 1 mL 10X Exonuclease I Buffer [670 mM Glycine-KOH, pH 9.5, 10 mM DTT, 67 mM MgCl2].
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