Takara
login | register | order now | view cart | feedback
Products
Molecular Biology


Products >  Molecular_Biology >  Modifying_Enzymes >  Nucleases >  Cryonase_Cold-Active_Nuclease

Cryonase Cold-Active Nuclease

Cryonase Cold-Active Nuclease is a recombinant endonuclease originally derived from a psychrophile, Shewanella sp., and purified from E. coli. This endonuclease enzyme can complete digestion of all types of DNA and RNA (single-stranded, double-stranded, linear or circularized) at cold temperatures and even in samples placed on ice. As a result, Cryonase Cold-Active Nuclease is frequently used during DNA digestion of mixed samples, such as those that include heat-labile proteins.

At-A-Glance Documents Images & Data

Applications

  • DNA digestion of all types of DNA
  • RNA digestion of all types of RNA
  • Enzymatic and other heat-labile reactions

Source

Purified from E. coli

Purity

  • Protease activity is less than the detection limit.
  • E. coli genomic DNA contamination is less than the detection limit.

Storage

–20°C

Buffer

Supplied in a buffer [20 mM NaCl, 10 mM Tris-HCl (pH 7.5), 50% glycerol, 2mM MgCl2]

References

1. Yang, H. et al. (2012) J. Virol. 86(23):13122-13123.

2. Baldermann, S. et al. (2010) J. Exp. Bot. 61(11):2967-2977.

Unit Definition

One unit is defined as the amount of enzyme required to increase 260 nm absorbance by 0.001 per minute at 37°C and pH 7.5 using salmon sperm DNA as the substrate.




 
 
Products
Cat. # Product Package Size Price License # of Units Select
2670A Cryonase™ Cold-Active Nuclease 10,000 Units $78.00 License Statements
2670B Cryonase™ Cold-Active Nuclease 50,000 Units $312.00 License Statements
 

 


Clontech is a Takara Bio Company © 2014 Privacy Policy | Terms & Conditions