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Products >  Molecular_Biology >  Modifying_Enzymes >  Nucleases >  Cryonase_Cold-Active_Nuclease

Cryonase Cold-Active Nuclease

Cryonase Cold-Active Nuclease is a recombinant endonuclease originated from a psychrophile, Shewanella sp., which is purified from E. coli. This enzyme can digest all types of DNA and RNA (single-stranded, double-stranded, linear or circularized) even on ice. Cryonase is useful for digestion of nucleic acids in samples, including heat unstable substances such as proteins.

At-A-Glance Documents

Applications

  • Digestion of all types of RNA and DNA

Source

Purified from E. coli

Purity

  • Protease activity is less than detection limit.
  • The contamination of E. coli genome DNA is less than detection limit.

Storage

–20°C

Form

20 mM NaCl 10 mM Tris-HCl, pH 7.5
50% glycerol 2 mM MgCl2

Unit Definition

One unit is the amount of the enzyme that increases the absorbance at 260 nm by 0.001 per minute at 37°C and pH 7.5, with salmon sperm DNA as a substrate.




 
 
Products
Cat. # Product Package Size Price License # of Units Select
2670A Cryonase™ Cold-Active Nuclease 10,000 Units $76.00 License Statements
2670B Cryonase™ Cold-Active Nuclease 50,000 Units $306.00 License Statements
 

 



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